3,4-Dichloroisocoumarin

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CAS: 51050-59-0

Name:

3,4-Dichloroisocoumarin

Other names:

3,4-Dichloro-1H-isochromen-1-one; 3,4-Dcl; 3,4-DCI; 3,4-Dichloro-2-benzopyran-1-one; 3,4-Dichloro-1H-2-benzopyran-1-one

3,4 Dichloroisocoumarin is a potent, irreversible inhibitor of serine proteases. It reacts with serine proteases to release an acylchloride moiety that can acylate another active site residue. 3,4 Dichloroisocoumarin also inhibits granzymes A, B, and H, cathepsin G, neutrophil elastase, proteinase and blocks apoptotic internucleosomal DNA cleavage in thymocytes without the involvement of endonucleases. 3,4 Dichloroisocoumarin inhibits activation of neutral sphingomyelinase and daunorubicin-triggered apoptosis as well as apoptosis induced by camptothecin in HL-60 cells. 3,4 Dichloroisocoumarin inhibits migration of eosinophils through basement membrane components in vitro. 3,4 Dichloroisocoumarin does not affect thiol proteases and metalloproteases. 3,4 Dichloroisocoumarin does not exhibit any activity towards β-lactamases. 3,4 Dichloroisocoumarin is an inhibitor of Chymotrypsin and Trypsin.

Interactions

Description

UniProt ID

Protein: Heat shock factor protein 1, Gene: HSF1
Protein: Granzyme B, Gene: GZMB
Protein: Chymotrypsin-like elastase family member 1, Gene: CELA1
Protein: Neutrophil elastase, Gene: ELANE
Protein: Complement factor D, Gene: CFD
Gene: Mcpt1l1
Protein: Chymase, Gene: CMA1
Protein: Myeloblastin, Gene: PRTN3
Protein: Cathepsin G, Gene: CTSG
Gene: SMPD1
Protein: Glycogen phosphorylase, muscle form, Gene: PYGM
Protein: Prostaglandin F2 receptor negative regulator, Gene: PTGFRN
Protein: Granzyme A, Gene: GZMA
Protein: Coagulation factor XII, Gene: F12
Protein: Complement C1r subcomponent, Gene: C1R
Protein: Mast cell protease 1, Gene: Mcpt1
Protein: Coagulation factor VII, Gene: F7
Protein: Chymotrypsin-like elastase family member 3B, Gene: CELA3B
Protein: Chymotrypsin-like elastase family member 3A, Gene: CELA3A
Protein: Chymotrypsin-like elastase family member 2A, Gene: CELA2A

Toxicity

oral LD50 [mouse] mg/kg
Unavailable
oral LD50 [rat] mg/kg
Unavailable
oral LD50 [rabbit] mg/kg
Unavailable

Effects on organism

No data
Antibacterial

No

Antifungal

No

Antiviral

No

Longevity mechanisms activation

Stress Resistance
+

Suppression of aging mechanisms

No data

Relation to biomarkers of Aging

Data not available

Relation to aging associated genes

No data available

Experimental conditions

S-complete media in wells of 96 well + E. coli OP50plates containing E. coli OP50

Life Extension

Mean LS (%)
13.0
Median LS (%)
Mortality rate derease (%)
Max LS (%)
Cell CLS
Cell RLS

Concentration wth maximum effect

0,33%

More info about experiment

24134630
Opposite effectNo